4a. Training Request & Licensing for BD instruments AMREPFlow core flow cytometry facility

Please do not initiate training steps 1-4 until you are ready to complete ALL aspects of training, including instrument induction, instrument training, and both licensing steps (with beads and with a sample of your own). The process of commencing from step 1 through to licensing conclusion should be completed within a month

Licensing step 4a. Contact [email protected] to request access to the AMREP Flow Induction Video. Make sure to take robust notes during this video as you will be asked to reproduce the steps at your first one on one session. If you don't bring robust notes at the first one on one session it will be cancelled.

Licensing step 4b. Now that you have completed steps 1-3 we can commence your practical training.

Please discuss with your supervisor which instrument will best suit your experimental needs.

Then email a request for your first practical training session. Our email is [email protected]

Please provide a few different times when you are available so that we can organise a convenient time that works for everyone.

The training sessions will prepare you for performing a 4 colour experiment on the instrument of your choosing.

The final step (the licensing) requires that you are able to perform:

1) fluidics start-up

2) navigate the acquisition software, generate worksheets and set gates

3) set baseline voltages

4) compensate using single colour controls

5) clean and shutdown the instrument

You are considered "licensed" when these steps are performed correctly without any help.

You will then be added to our Bookings website and issued a log-in so that you can book instruments 24/7 and commence your flow experiments on your own.

We look forward to meeting and training you.

The AMREPFlow team