Access prerequisites AMREPFlow core flow cytometry facility
Bookings
Bookings can be made via the AMREP Flow booking sheet. Please be aware of the following:
If you are planning on using and performing regular immunophenotyping on primary human samples on any of the AMREPFlow instruments, the sample MUST be fixed. (Fixation protocol under instrument SOPs and Suggested Protocols - Education tab)
If you require to run and analyze primary human samples that are NOT FIXED for functional/kinetic etc. studies, you MUST submit a Risk Assessment Document and an SWI to AMREPFlow, as the study will have to be approved by the AMREPFlow OHS Committee. Once you attain your OHS approved project number:
- Before commencing your run, please cover the keyboard and mouse with glad wrap
- Upon completion of the work, remove and discard glad wrap into yellow bins
- Wipe down workbench areas, keyboard and mouse with 80% v/v Ethanol or 5% Bleach solution.
Thank you for your cooperation and for making our/your working environments safer.
All samples MUST be filtered (minimum 100um cell strainer) before being run on any instrument to prevent blockages.
Please ensure you have the correct sample concentration prior to sorting - 1 x 10^7 cells / mL
Bookings for all analysis instruments can be made right up until the time of the booking & can be cancelled up until 15 hours before the required session.
Bookings for the Fusion, InFlux & ARIA sorters must be made more than 24 hours before the intended sort time. If you require a sorter booking within 24 hours please contact AMREP Flow to ensure availability.
Cancellation of bookings on the Fusion, InFlux and ARIA sorters can be up to 72 hours before your booking. If you do not turn up to your booking you will still be charged. Last minute cancellations will only be exempt from charge in extreme circumstances. Please contact us in the case of any last minute cancellation.
If you want to cancel within 72 hours of your booking, you may find someone else to fill your spot from your own contacts. AMREP Flow staff will assist where they are able to, but will not be responsible for pro-actively filling that time.
Fusion, Influx and ARIA sorters are unavailable on all public holidays, including non-Monash observed: Labour Day, Queens Birthday & Melbourne Cup Day. Bookings on these days will not be honoured.
Please respect the booking sheet. Unbooked use of instruments will not be tolerated and those caught abusing the system risk having access revoked.
There is to be NO food or drink in any Flow Cytometry laboratory. Please observe PC2 requirements at all times.
Cancellation policy
We understand that sometimes experiments fall through and have to be cancelled. Self cancellations can be made on Analysers up to 15 hrs prior to the experiment and on the Sorters up to 72 hrs prior to the sort booking. These cancellation windows allow for others to utilize sort and analysis time. For short notice cancellations on analysers (within 15hrs) or sorters (within 72hrs) contact us by email [email protected] or phone (99030601). We don't make cancellations for no-shows, early finishes or experiment procedural mistakes. However, a booking may be cancelled if either of the follow two exceptions apply; a clinical patient sample no-show or if the researcher is away with emergencies/illness.
